Alaa Khedr, Mohamed Moustafa, Ashraf B. Abdel-Naeim, Abdulrahman Alahdal, and Hisham Mosli.
High-Performance Liquid Chromatographic Method for Determination of Phenytoin in Rabbits Receiving Sildenafil, Analytical Chemistry Insights 2008:3 61–67A validated high-performance liquid chromatographic (HPLC) method for determination of phenytoin (PHN), para-hydroxy metabolite of phenytoin (POH) and sildenafil (SIL) in rabbit plasma is described. The method is based on extraction on Sep-Pak C18 solid support using ethylacetate and ether as eluents and monitoring at 220 nm.