ChemSpider 2D Image | DAPI | C16H17Cl2N5

DAPI

  • Molecular FormulaC16H17Cl2N5
  • Average mass350.246 Da
  • Monoisotopic mass349.086090 Da
  • ChemSpider ID140783

More details:

Validated by Experts, Validated by Users, Non-Validated, Removed by Users

1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, hydrochloride (1:2) [ACD/Index Name]
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
2-(4-Carbamimidoylphenyl)-1H-indol-6-carboximidamiddihydrochlorid [German] [ACD/IUPAC Name]
2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride [ACD/IUPAC Name]
2-(4-Carbamimidoylphényl)-1H-indole-6-carboximidamide, dichlorhydrate [French] [ACD/IUPAC Name]
249-186-7 [EINECS]
28718-90-3 [RN]
4',6-Diamidino-2-phenylindole dihydrochloride
DAPI [Wiki]
DAPI (hydrochloride)
More...

Validated by Experts, Validated by Users, Non-Validated, Removed by Users

4894417 [DBID]
32670_FLUKA [DBID]
D8417_SIGMA [DBID]
D9542_SIGMA [DBID]
  • Experimental Physico-chemical Properties

    • Experimental Solubility:

      Soluble in water (10mg/ml, gentle warming), and in methanol Hello Bio HB0747
      Soluble to 100 mM in DMSO and to 10 mM in water with gentle warming Tocris Bioscience 5748
  • Miscellaneous

    • Bio Activity:

      <p><span style="text-decoration: underline;"><strong>Overview</strong></span></p> <p>DAPI is a blue fluorescent DNA stain which is cell permeant at high concentrations.</p> <p style="margin-bottom: 2em;">DAPI binds strongly to A-T rich regions in DNA to form a fluorescent complex. It preferentially stains ds-DNA and has a high quantum yield (?f=0.92) when bound to DNA.</p> <p><strong><span style="text-decoration: underline;">Uses and applications</span></strong></p> <p>DAPI is commonly used as a nuclear and chromosome counterstain.</p> <p>It is preferentially used to stain dead cells. DAPI is less effective as a live cell stain as it is unable to efficiently pass through the membrane in live cells. Therefore, higher concentrations may need to be used.</p> <p style="margin-bottom: 2em;">Cells must be permeabilized and/or fixed for DAPI to enter the cell and bind to DNA.</p> <p style="margin-bottom: 2em;">Due to DAPI’s blue emission, there is very little fluorescent overlap between yellow-fluorescent, green-florescent molecules (e,g, fluorescein and GFP) or red-fluorescent stains (e.g. Texas red). It is therefore convenient for multiplexing assays.</p> <p>DAPI has a great variety of applications but is often used for cell imaging, cell counting, cell sorting (based on DNA content), apoptosis analysis and in HCA (high-content analysis).</p> <p> </p> <p>DAPI Staining Solution (1mg/mL) also <a title="DAPI Staining Solution (1mg/mL) | Hello Bio" href="/dapi-ready-made-staining-solution.html">available</a>.</p> Hello Bio HB0747
      <p><span style=text-decoration: underline;><strong>Overview</strong></span></p> <p>DAPI is a blue fluorescent DNA stain which is cell permeant at high concentrations.</p> <p style=margin-bottom: 2em;>DAPI binds strongly to A-T rich regions in DNA to form a fluorescent complex. It preferentially stains ds-DNA and has a high quantum yield (&phi;f=0.92) when bound to DNA.</p> <p><strong><span style=text-decoration: underline;>Uses and applications</span></strong></p> <p>DAPI is commonly used as a nuclear and chromosome counterstain.</p> <p>It is preferentially used to stain dead cells. DAPI is less effective as a live cell stain as it is unable to efficiently pass through the membrane in live cells. Therefore, higher concentrations may need to be used.</p> <p style=margin-bottom: 2em;>Cells must be permeabilized and/or fixed for DAPI to enter the cell and bind to DNA.</p> <p style=margin-bottom: 2em;>Due to DAPI&rsquo;s blue emission, there is very little fluorescent overlap between yellow-fluorescent, green-florescent molecules (e,g, fluorescein and GFP) or red-fluorescent stains (e.g. Texas red). It is therefore convenient for multiplexing assays.</p> <p>DAPI has a great variety of applications but is often used for cell imaging, cell counting, cell sorting (based on DNA content), apoptosis analysis and in HCA (high-content analysis).</p> Hello Bio HB0747
      <p>Blue fluorescent DNA stain. Nuclear counterstain.</p> Hello Bio HB0747
      Biochemicals & small molecules Hello Bio HB0747
      Fluorescent DNA stain Tocris Bioscience 5748
      Fluorescent DNA stain. Binds to AT-rich regions of DNA. Cell permeable at high concentrations. Tocris Bioscience 5748
      Fluorescent Dyes Tocris Bioscience 5748
      Fluorescent Dyes, Labels and Stains Tocris Bioscience 5748
      Fluorescent Imaging Tocris Bioscience 5748
      Imaging, dyes, stains & labels/DNA fluorescent stains Hello Bio HB0747
      Reagents Tocris Bioscience 5748

Predicted data is generated using the ACD/Labs Percepta Platform - PhysChem Module, version: 14.00

No predicted properties have been calculated for this compound.

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